Milk Samples for Udder Health

Collecting Samples from Individual Cows 

Please use the following steps to avoid contamination :

  • It is preferable for the collector to wear latex or vinyl gloves.  If gloves are not worn, the collector's hands must be clean and dry.
  • The person collecting the samples should not be milking at the same time.
  • Samples should be taken directly form the teats.  Bucket or milk meter samples tend to carry over bacteria from the previous cow
  • The sample should be taken before the cow is milked.  If the sample is taken during midday, it should be taken at least 4 hours after the last milking.
  • Samples tubes should be handled properly to ensure sterility at all times.  The collection tube should be held at an angle (approximately 45 degree).  Do not put caps into pockets, touch the tops or touch the inside of the collection stubs.  Avoid getting particles of dust, dirt or manure into the sample tube.
  • The most important factor is that teats need to be clean and dry. Prep the cow as usual, but teats need to be dried completely by the person doing the sample.
  • After the routine teat preparation, scrub the teat ends thoroughly with alcohol swab or sterile cotton ball saturated with alcohol.  Clean the teats on the far side of the udder first.  Be careful not to touch teat ends before taking the milk sample. Sample the teats closest to you first.  Discard a squirt or tow of milk before sample collection
  • While holding the tube at an angle collect a squirt two of milk from each quarter being the sampled.  If collecting milk from all quarters (composite sample) try and take the same amount of milk from each quarter.
  • Open barn doors or tunnel ventilation can cause massive air movement, resulting in major contamination problems from bedding and dust.  Feeding during sampling should be avoided.
  • Tighten the cap and properly label the collection tube using a fine tip water proof marker.  Immediately place in a refrigerator or freezer.  Keep cold or frozen until delivery to the our laboratory.  Bacteria in samples that are not cooled or frozen may grow, resulting in misleading information.
Courtesy of MVDL

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