|
PROCEDURE
|
SPECIMEN
|
CONTAINER
|
SHIPPING
(refrigeration requirements)
|
LAB TIME
|
COMMENTS
|
|
Acid
Fast Stain
|
Ileum,
lymph node, rectal mucosa scraping; tissue impression smear, feces
|
Sterile
container for tissue, slide folder for
slides
|
cold
|
Same day
|
Submit 3
slides when submitting tissue impression smears or rectal mucosal
scrapings.
|
|
Bacterial
aerobic culture
|
Affected
tissue, swab, exudate or milk
|
Sterile
container, culturette, with Amies transport media
|
cold
|
3-5 days
|
Identify
source site for swabs. Milk
aseptically collected. A least
2ml of milk required
|
|
Bacterial
anaerobic culture
|
Tissue;
swab
|
Anaerobic
transport culturette; sterile container
|
cold
|
7-10 days
|
Identify
site for swabs & exudates; animals dead for more than 4-hours are
generally unsuitable for any anaerobic cultures due to invading
post-mortem invasion of organisms. |
|
Antimicrobial
susceptibility
|
Bacterial
isolate
|
Bain
heart infusion slant
|
cold
|
2 days
|
Infectious substance
shipping required
|
|
Anthrax
|
Blood
, spleen, eyeball, ocular fluid
|
Red top
vacutainer tube for blood or eyeball fluid, sealed container, spleen or
eyeball.
|
cold
|
2-5 days
(prelim. same day)
|
Label
submitting paper work: Anthrax
Suspect; Contact lab before submitting; do NOT
send umbilical blood tape
|
|
Brucella
Culture
|
Tissue;
milk- at least
20ml/ quarter, 100ml composite
|
Sterile
container
|
cold
|
7-14 days
|
Contact
lab before submitting. Collect Aseptically.
|
|
Campylobacter
fetus fetus
(abortions in sheep and occ. cattle)
|
Stomach
contents; fetus; fetal membranes; Placenta
|
Clark’s
or Amies w/charcoal transport media
for washes & swabs. Sterile container for tissues
|
cold
|
7 days
min.
|
Samples
should reach the lab within 24 hours of collection, in the absence of
placenta the likelihood of a diagnosis is severely reduced.
|
|
Campylobacter
fetus jejuni
(diarrhea)
|
Freshly
passed feces
|
Sterile
container
|
cold
|
7 days
min.
|
Samples
submitted in gloves/OB sleeves will be refused; Ideally samples should reach the
lab within 5 hours of collection
|
|
Campylobacter
fetus venerealis
(infertility)
|
Vaginal
mucus (from heifer 30-80 days post-breeding); Preputial washing (from
bull)
|
Amies
transport media (call ahead to obtain from lab)
Amies transport media (call ahead to obtain from lab)
|
cool
|
7 days
min.
|
Washing
must be cloudy and blood tinged to show adequate sampling; Do not
refrigerate; Transport immediately to lab, must be maintained in
microaerophilic atmosphere to survive; Samples should reach the lab within
5 hours of collection
|
|
Campylobacter
hyointestinalis
(Swine Proliferative Enteritis)
|
Affected intestine
|
. Sterile
container
|
cold
|
7 day min.
|
Samples
should reach the lab within 5 hours of collection
|
|
Campylobacter
sputorum mucosalis
|
Affected intestine
|
Sterile
container
|
cold
|
7 days
min.
|
Samples
should reach the lab within 5 hours of collection
|
|
Clostridial
FA*
|
Deep
muscle tissue
|
Sterile
container
|
cold
|
Same day
|
Specimen
must arrive at the lab within 24 hours of collection; Cl. septicum is a
frequent post-mortem invader.
|
|
Dermatophilus
(Dermatophilosis)
|
Thick
scabs
|
Sterile
container
|
cold
|
3-5 days
(prelim. 24hrs)
|
Use
leakproof containers
|
|
Fungal
Culture
|
Hair
with root (for dermatophytes);. Scab;Tissue (for systemic mycoses);. Fluid (for systemic
mycoses)
|
Sealed
paper envelope; Sterile container
|
cold
|
14-30
days.
|
Samples
for dermatophyte testing must NOT be submitted in an airtight container;
Samples sent with clipped hair are unacceptable; Good collection technique
is ESSENTIAL for proper culture results.
COLLECTION TECHNIQUE (for dermatophytes): First CLEAN the area well with
alcohol, allow to dry, then collect sample, plucking hair and scraping the
edge of lesion; Samples submitted without first disinfecting the area will
usually be overgrown by contaminants, resulting in a false negative
culture.
|
|
Gram
Stain
|
Slide Impression of
Tissue; heat fix
|
Slide
folder; Culture tube (or sealed plate)
|
cold
|
Same day
|
For entrotoxemia, estimates
of clostridium, intestine smears should
be made within 1-2 hrs postmortem.
|
|
Milk
Bulk Tank Sample
(for quantitative analysis)
|
Representative
sample
|
Sterile
screw cap or snap top vial
|
cold
|
3-5 days
|
Freeze IMMEDIATELY
following collection; screw cap tubes are available for purchase from the
lab
|
|
Milk
Culture
|
Quarter or
combined sample
|
Sterile
screw top or snap top tube
|
cold
|
3-5 days
|
Sterile Collection
|
|
Mycobacterium*
paratuberculosis
(Johne's)
|
Fresh
feces (at least 3gms, thumb size lump);.intestine (ileocecal junction,
ileum, and adjacent lymph nodes are best)
|
Sterile
container or whirlpacks
|
cold
|
4 months
|
Samples submitted in inverted
OB
sleeves, gloves, or open
containers are NOT acceptable; and will be refused; Immediately refrigerate
tissue samples. Culture held 15 weeks before considered negative.
|
|
Mycoplasma
Culture
(bovine only)
|
Milk;
Swab; Fresh tissue
|
Sterile
screw top or snap top tube; Amies with charcoal or culturette. Sterile
container for milk
|
cold
|
7-21 days
|
Contact
laboratory before submitting to insure media is available.
|
|
Urine
Culture
|
Urine
collected by cystocentesis or catheter
|
Sterile
leakproof container
|
cold
|
3 days
|
Urine
samples collected by cystocentesis or from catheterized animals are
preferred, however, mid-stream collections are suitable.
|
